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Chemicals for Diagnostic Tests and Clinical Products
Thermo Scientific™ DpnI (10 U/µL) Thermo Scientific™ ER1701
Availablity:
In stock
Catalog/Part Number:
10669500
Brand:
THERMOFISHER SCIENTIFIC
Supplier:
FISHER SCIENTIFIC
Supplier Location:
LOUGHBOROUGH, UK
Inventory Weight:
unavailable
Unit of Measurement:
Inventory Brochure:
unavailable
Catalog/Part Number:: 10669500
₦66,240.00
Description
Highlights
Superior
quality – stringent quality control and industry leading manufacturing process
Convenient
color-coded Five Buffer System
Includes
universal Tango buffer for double-digestions
BSA premixed
in reaction buffers
Wide
selection of restriction endonuclease specificities
Applications
Molecular
cloning
Restriction
site mapping
Genotyping
Southern
Blot
Restriction
fragment length polymorphism (RFLP)
SNP
Note
DpnI
requires the presence of N6-methyladenine within the recognition sequence to
cleave DNA. DNA purified from a dam+ strain will be a substrate for DpnI. DpnI
will only cleave fully-adenomethylated dam sites. Hemi-adenomethylated dam
sites DpnI cleaves 60X more slowly. DpnI, Bsp143I, and MboI all recognize the
same sequence, but have different methylation sensitivities and cleavage sites.
Assayed using pBR322 DNA.
Technical Specifications
| Product Type | DpnI |
| Concentration | 10U/μL |
| Incubator Temperature | 37°C |
| Quantity | 500U |
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