Comprehensive Two-Dimensional Liquid Chromatograph

Description

Novel Separation Method for Complicated Sample Matrices

The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial to the analysis of pharmaceutical impurities, proteolytic digests, food extracts, natural products and synthetic polymers.

The Nexera-e incorporates a Shimadzu designed low-volume switching valve. Comprehensive 2D-LC requires frequent valve switching. Some valve systems may exhibit clogging issues due to abrasion powders. Using the robust technology developed for the Nexera X2 autosampler, this potential problem has been significantly minimized. Ultra-high speed analysis of comprehensive 2D-LC also requires ultra-high-speed data sampling. The Nexera X2 photo-diode array detector, SPD-M30A, has a high sampling rate up to 200 Hz, while the LCMS-8050 triple quadrupole liquid chromatograph mass spectrometer features ultra-high speed data scanning by employing UF-MS technologies to enable superior data acquisition.