Thermo Scientific™ TAE Buffer (Tris-acetate-EDTA) (50X)

Description

Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).

Usage Recommendations

Use fresh 1X TAE both for the gel and for the electrophoresis run

To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well

Quality Control

The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

40mM Tris

20mM acetic acid

1mM EDTA

pHof 50X TAE: 8.4

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.

Note:

TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.