Sorry no inventory found for this brand at this time.
Enzymes and Inhibitors
Thermo Scientific™ FastDigest Value Pack Thermo Scientific™ ER0971
Availablity:
In stock
Catalog/Part Number:
10704111
Brand:
THERMOFISHER SCIENTIFIC
Supplier:
FISHER SCIENTIFIC
Supplier Location:
LOUGHBOROUGH, UK
Inventory Weight:
unavailable
Unit of Measurement:
Inventory Brochure:
unavailable
Catalog/Part Number:: 10704111
₦57,960.00
Description
The NheI restriction enzyme recognizes G^CTAGC sites and cuts best at
37°C in Tango buffer (Isoschizomers: AsuNHI, BmtI).
5'...G▵G W C C...3'
3'...C C W G▵G...5'
Conditions for 100% Activity:
1X Buffer R:10mM Tris-HCl (pH 8.5 at 37°C), 10mM MgCl2, 100mM KCl and
0.1mg/mL BSA
Incubate at 37°C
Storage Buffer:
Eco47I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM
DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage:
After 50-fold overdigestion with Eco47I, more than 95% of the DNA
fragments can be ligated and recut
Methylation Effects:
Dam: never overlaps-no effect
Dcm: may overlap-blocked
CpG: may overlap-blocked
EcoKI: never overlaps-no effect
EcoBI: never overlaps-no effect
Compatible Ends:
Cfr13I, CpoI, Psp5II, SanDI
Note:
Eco47I is blocked by overlappingdcm methylation. To avoiddcm
methylation, use adam,dcm- strain such as GM2163 (#M0099).
Technical Specifications
Highlights
Superior
quality – stringent quality control and industry leading manufacturing process
Convenient
color-coded Five Buffer System
Includes
universal Tango buffer for double-digestions
BSA premixed
in reaction buffers
Wide
selection of restriction endonuclease specificities
Applications
Molecular
cloning
Restriction
site mapping
Genotyping
Southern
Blot
Restriction
fragment length polymorphism (RFLP)
SNP
Note
Low salt,
high glycerol (> 5%) concentrations, pH > 8.0 or a large excess of enzyme
may result in star activity. NheI is inhibited by salt concentrations above 100
mM. Supercoiled plasmids may require up to 10-fold more NheI for complete
digestion than linear DNAs (e.g. 10 units are required to cleave 1 µg of.)
Zero(0) Reviews