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Enzymes and Inhibitors
Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA)
Availablity:
In stock
Catalog/Part Number:
10399519
Brand:
THERMOFISHER SCIENTIFIC
Supplier:
FISHER SCIENTIFIC
Supplier Location:
LOUGHBOROUGH, UK
Inventory Weight:
unavailable
Unit of Measurement:
Inventory Brochure:
unavailable
Catalog/Part Number:: 10399519
₦22,770.00
Description
Usage Recommendations
- Use fresh 1X TAE both for the gel and for the electrophoresis run
- To prepare 1X TAE buffer add 20ml of 50X TAE buffer to 980ml of deionized water and mix well
Quality Control
- The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests
1X Composition
- 40mM Tris
- 20mM acetic acid
- 1mM EDTA
- pHof 50X TAE: 8.4
Recommended for:
Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for resolution of RNA and DNA fragments l.
Note:
TAE buffer has a relatively low buffering capacity, therefore periodic replacement of the buffer during prolonged electrophoresis is recommended.
Technical Specifications
|
Product Type |
50X TAE Buffer
(Tris-acetate-EDTA) |
|
Components |
40mM Tris; 20mM
acetic acid; 1mM EDTA; pHof 50X TAE: 8.4 |
|
Quantity |
1L |
|
Storage Requirements |
There are no time
limitations for storage of the electrophoresis buffers at room temperature.
If the buffer is stored at lower temperatures, a precipitate may form, which
is easily dissolved by gentle heating. |
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