Thermo Scientific™ 10X TBE Buffer (Tris-borate-EDTA)

Description

Usage Recommendations

• Use fresh 1X TBE both for the gel and for the electrophoresis run
• To prepare 1X TBE buffer, add 100mL of 10X TBE buffer to 900mL of deionized water and mix well

Quality Control

• The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests

1X Composition

• 89mM Tris, 89mM boric acid, 2mM EDTA, pH of 10X TBE: 8.3

Recommended for:

Electrophoresis of nucleic acids in agarose and polyacrylamide gels; Used both as a running buffer and as a gel preparation buffer; Filtered through a 0.22μm membrane; Recommended for electrophoresis of RNA and DNA fragme.

Note:

Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.