Iwai North America ALyS505NK-AC: Xeno-free (Serum free) cell culture mediums

ALyS505NK-AC is xeno-free cell culture medium without IL-2, optimized for human natural killer (NK) cells derived from peripheral blood mononuclear cells (PBMCs). ALySNK-AC demonstrates a remarkable capability to increased collection of NK cells, coupled with heightened cytotoxicity. Unlike the majority of NK-cell culture kits, ALyS NKs unique medium composition establishes optimal conditions for NK cells, leading to a significant increase in NK cell percentage, typically ranging from 60% to 80%. By employing ALyS505NK-AC for activation and ALyS505NK-EX for expansion, one can attain a highly pure, NK-rich population of cells in 14 days without the need for sorting with beads or flow cytometry. 1. Mikawa, S., Matsuda, A., Kamemori, Y., Asanuma, S. & Kitagawa, H. Enhancement of natural killer cell activity by oral administration of a fermented soybean product in dogs. Open Veterinary Journal 11, 394–400 (2021) doi: 10.5455/OVJ.2021.v11.i3.10.