Gibco™ DMEM/F-12, GlutaMAX™ Supplement
Catalog/Part Number:: 11559726
₦45,540.00
Description
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEMF/12, GlutaMAX™ Supplement is used in cell culture processing applications.
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Technical Specifications
For Use With (Application): Mammalian cell culture
Formulation: Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline
chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose
(Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic
Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Alanyl-L-Glutamine, L-Arginine
hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O,
L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine,
L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline,
L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate,
L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium
Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl),
Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate
(NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous,
Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine
hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
Product Type: DMEM/F-12 media
Storage Requirements: Storage: 2-8° C. Protect from light
Without Additives: HEPES
Format: Liquid
Packaging: Sustainable packaging
Quantity: 500mL
With Additives: GlutaMAX™ Supplement, Phenol Red
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