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Cell Culture Media
Gibco™ DMEM/F-12
Availablity:
In stock
Catalog/Part Number:
11554546
Brand:
GIBCO
Supplier:
FISHER SCIENTIFIC
Supplier Location:
LOUGHBOROUGH, UK
Inventory Weight:
unavailable
Unit of Measurement:
Inventory Brochure:
unavailable
Catalog/Part Number:: 11554546
₦35,190.00
Description
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM/F-12 is used in cell culture processing applications.
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Technical Specifications
For Use With (Application): Mammalian cell culture
Formulation: Biotin, Calcium Chloride (CaCl2) (anhyd.),
Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate,
D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate
(FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine Na, L-Alanine, L-Arginine
hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O,
L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O,
L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine,
L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt
dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride
(anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red,
Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride,
Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium
Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic
(NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12,
Zinc sulfate (ZnSO4-7H2O), i-Inositol
Product Type: DMEMF/12 media
Storage Requirements: Storage: 2°C to 8°C. Protect from
light
Without Additives: HEPES
Format: Liquid
Packaging: Sustainable packaging
Quantity: 500mL
With Additives: L-Glutamine, Phenol Red
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