Gibco™ DMEM, High Glucose, No Glutamine
Catalog/Part Number:: 11500416
₦43,470.00
Description
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, No Glutamine is used in cell culture processing applications.
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Technical Specifications
For Use With (Application): Mammalian Cell Culture
Formulation: Calcium Chloride (CaCl2) (anhyd.), Choline
chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate
(Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl,
L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride,
L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine
disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.),
Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride,
Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium
Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Product Type: DMEM media
Storage Requirements: Storage: 2-8° C. Protect from light
Without Additives: L-glutamine; Sodium Pyruvate; HEPES
Format: Liquid
Packaging: Sustainable packaging
Quantity: 500mL
With Additives: High Glucose; Phenol Red
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