Gibco™ DMEM, High Glucose 11330882
Catalog/Part Number:: 11330882
₦347,760.00
Description
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose is used in cell culture processing applications.
- Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12.
Technical Specifications
For Use With (Application): Antibody and cell culture production,
Mammalian cell culture
Formulation: Calcium Chloride (CaCl2) (anhyd.), Choline
chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate
(Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Arginine hydrochloride, L-Cystine 2HCl,
L-Glutamine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine
hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine,
L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate
(MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine
hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl),
Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, i-Inositol
Product Type: DMEM media
Storage Requirement: Storage: 2-8° C. Protect from light
Without Additives: Sodium Pyruvate, HEPES
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